
Becke' Line Dispersion Staining
Transmitted Brightfield, Closed SubStage Condenser Iris, 20X Objective
These are the colored Becke' lines seen with the 1.67 optical glass standard in Cargille Melt Mount 1.662 refractive
index liquid when the distance between the slide and the objective is increased. The wavelength at which this glass
and this liquid match is 490 nanometers. The difference in dispersion is 0.011.