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Dispersion Staining, Becke' Line
Becke' Line Dispersion Staining was first documented in 1911 by F. E. Wright in Publication #158 by the Carnegie Institute. He noted that when a particle and a liquid matched refractive indices for some wavelength in the visible range but they had different dispersion curves then two Becke' lines would be formed on defocusing the particle. The Becke' line for the blue end of the spectrum would move into the material with the steepest dispersion curve and the Becke'line for the red end of the spectrum would move into the material with the flatest dispersion curve.